Solution Reagent Kit

The AmpliSeq for Illumina Immune Response Panel is a targeted resequencing research assay that quantifies the expression of cancer biomarkers in 395 genes that are involved in the interactions between the immune system and the tumor.input:
output:Panel characteristicsinput:
output:Relevant Gene Content - Targeted expression of 395 cancer biomarkers that are indicative of immunotherapy response.Rapid, Efficient Process - In a single day, sequencing-ready libraries can be generated from as little as 1 ng of high-quality RNA or 10 ng of formalin-fixed, paraffin-embedded (FFPE) RNA input samples.input:
output:
Accurate Data - Identify the expression of genes that are involved in the interactions between the immune system and tumors.The Immune Response Panel is a component of an integrated RNA-to-annotated variant solution that encompasses automated analysis, sequencing by synthesis (SBS) next-generation sequencing (NGS) technology, and AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation.Specificationsinput:
output:Assay time: 6 hours (library preparation only; does not include library quantification, normalization, or pooling time)Content specifications - 395 genes that are linked to the immune responseinput:
output:Minimum 1.5 hours of hands-on experienceinput:
output:Input quantity: 1–100 ng (10 ng is recommended per pool)input:
output:Method: Targeted RNA sequencing, amplicon sequencinginput:
output:Multiplexing - 96 dual index combinationsinput:
output:RNA is the type of nucleic acid.input:
output:In total, 398 amplicons are present in one pool.Sample types that are specialized include FFPE tissue.input:
output:Transcript variants are classified as a variant class.