Reverse Transcriptase Reagent
Applications | For Rt-Pcr, For Cdna Synthesis |
Type | Reverse Transcriptase |
Other Characteristics | Hot Start |
Convenient cDNA synthesis and PCR in a single tube from 1 pg of total RNA
Formulated for the highly specific and sensitive RT-PCR of any RNA template
Incorporates Hot-Start Taq and thermostable reverse transcriptase for room-temperature preparation.input:
output:The Accuris One-Step RT-PCR Kit has been designed to facilitate the synthesis of cDNA and the subsequent PCR in a single tube for end-point analysis. The Accuris Hot Start Taq, an advanced RNase Inhibitor, and an MMLV-derived, thermostable Reverse Transcriptase (45°C to 55°C) comprise this most recent generation RT-PCR Kit. It enables ultra-sensitive one-step RT-PCR from as little as 1pg total RNA starting material.input:
output:The optimized buffer chemistry enables the efficient reverse transcription and PCR of problematic sequences with significant secondary structure, such as GC-rich targets. The Accuris One-Step RT-PCR Kit is perfect for qualitative analysis of RNA transcription levels to quantify expression and determine the presence or absence of RNA templates. The kit also effectively synthesizes double-stranded cDNA for subsequent gene expression analysis.input:
output:PR1100-50 – Accuris One-Step RT-PCR kit, 50 reactions
PR1100-100 – Accuris One-Step RT-PCR kit, 100 reactions


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