Enzyme Reagent Kit

The mRNA cap structure 2′-O-methyltransferase, vaccinia virus capping enzyme, and other capping reaction components are present in this product. These components are capable of modifying the 5′ cap of RNA produced by T7 in vitro transcription (JT101).input:
output:In eukaryotes, a unique cap structure must be formed at the 5′ end after transcription to produce the original mRNA. The stability, transport (exit), and translation of mRNA are significantly influenced by this structure. The enzymatic reaction of capping enzymes to modify the 5′ end of RNA is a straightforward and efficient method: The 7-methylguanylate cap (m7Gppp, Cap0) can be attached to the 5′ end of RNA by the Vaccinia Capping Enzyme, resulting in the formation of m7Gppp5’N-mRNA (Cap0-mRNA). The mRNA Cap 2′-O-methyltransferas utilizes CAP0-mRNA as a substrate and SAM (S-adenosine methionine) as a methyl donor to methylate the 2′-OH of the first nucleotide of the 5′ end of CAP0-mRNA, which is adjacent to the cap structure, resulting in the formation of Cap1-mRNA. input:
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The Cap1 structure has the potential to enhance the expression ability of mRNA in cell transfection and microinjection experiments by improving mRNA stability.input:
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This product completes the capping reaction by simultaneously employing the vaccinia virus capping enzyme and mRNA Cap-2′-O-methyltransferase in the capping reaction. The capping efficiency can be nearly 100% when the cap is oriented correctly, and the reaction product is Cap1-mRNA.input:
output:Shippinginput:
output:Dry ice (-70 ℃)